PCR-RFLP assay as an option for primary HPV test
نویسندگان
چکیده
منابع مشابه
PCR-RFLP assay as an option for primary HPV test
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for ...
متن کاملA Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
Objective(s): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica , based on profiles of RFLP, produced by effect of endo...
متن کاملHPVTyper: A Software Application for Automatic HPV Typing via PCR-RFLP Gel Electrophoresis
The cervical cancer is currently the most prominent case of association between HPV and cancer. The persistent infection of the anogenital tract by a group of HPV types has been proved to be the main causal factor of cervical cancer (Bosch et al., 2002; Walboomers et al., 1999). This group of cervical cancer related types currently includes 30 to 40 types. However, not all the types in the grou...
متن کاملA Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species
OBJECTIVE(S) Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on I...
متن کاملA one-enzyme PCR-RFLP assay for identification of six medically important Candida species.
Early identification of Candida isolates to the species level is necessary for effective antifungal therapy, and can also facilitate control of hospital infections. Phenotype-based methods for identifying Candida species are often difficult and time-consuming. Molecular biological techniques provide a useful alternative approach. In the present study, the ITS1-5.8S-ITS2 regions of fungal rRNA g...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2018
ISSN: 1414-431X,0100-879X
DOI: 10.1590/1414-431x20177098